The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER

FEBS Lett. 2003 Apr 10;540(1-3):86-90. doi: 10.1016/s0014-5793(03)00229-1.

Abstract

Bcl-2/adenovirus E1B 19 kDa interacting protein 2-like, BNIP-2-like (BNIPL) is a recently cloned and characterized apoptosis-associated protein that shares 72% homology with BNIP-2. It is highly expressed in human placenta and lung. A yeast two-hybrid system was used to obtain two BNIPL-interacting proteins, MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1 (Saccharomyces cerevisiae)-like). The interactions were confirmed by glutathione S-transferase pull-down assay in vitro and co-immunoprecipitation assay in vivo. Colony formation assay and cell proliferation test suggest that overexpression of BNIPL could inhibit the growth of BEL-7402 cells. These findings suggest that BNIPL may physically bind to cell proliferation-related proteins, MIF and GFER.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins / metabolism*
  • Cell Cycle Proteins / metabolism*
  • Humans
  • Precipitin Tests
  • Protein Binding
  • Two-Hybrid System Techniques

Substances

  • Adaptor Proteins, Signal Transducing
  • BNIPL protein, human
  • Carrier Proteins
  • Cell Cycle Proteins