[Effect of 1,25-dihydroxyvitamin D3 and 17beta-estradiol on hpl-bp74 gene regulation in breast adenocarcinoma cell line]

Tsitologiia. 2003;45(1):69-73.
[Article in Russian]

Abstract

Data are provided on the upregulation of heterochromatin protein binding protein 74 gene (hpl-bp74) at RNA transcription level under the influence of stimulation of 1,25-dihydroxyvitamin D3. We also showed the absence of action of 17 beta-estradiol on the transcription level of hpl-bp74 breast adencarcinoma cell line (MCF7). The protein encoded by gene hpl-bp74 can be involved in generalized transcriptional regulation and thus take part in inhibition of proliferation by 1,25(OH)2D3. Computer analyses of DNA sequences of hpl-bp74 enabled us to identify some potential hormone responsive elements responsible for the binding of nuclear receptor for vitamin D with the DNA motifs.

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Calcitriol / pharmacology*
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / genetics*
  • Chromosomal Proteins, Non-Histone / metabolism
  • Estradiol / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Humans
  • RNA, Messenger / metabolism
  • Receptors, Calcitriol / metabolism
  • Regulatory Sequences, Nucleic Acid*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / metabolism
  • Transcription, Genetic*
  • Tumor Cells, Cultured
  • Vitamin D-Binding Protein / metabolism

Substances

  • Chromosomal Proteins, Non-Histone
  • RNA, Messenger
  • Receptors, Calcitriol
  • Transcription Factors
  • Vitamin D-Binding Protein
  • Chromobox Protein Homolog 5
  • Estradiol
  • Calcitriol