Abstract
Data are provided on the upregulation of heterochromatin protein binding protein 74 gene (hpl-bp74) at RNA transcription level under the influence of stimulation of 1,25-dihydroxyvitamin D3. We also showed the absence of action of 17 beta-estradiol on the transcription level of hpl-bp74 breast adencarcinoma cell line (MCF7). The protein encoded by gene hpl-bp74 can be involved in generalized transcriptional regulation and thus take part in inhibition of proliferation by 1,25(OH)2D3. Computer analyses of DNA sequences of hpl-bp74 enabled us to identify some potential hormone responsive elements responsible for the binding of nuclear receptor for vitamin D with the DNA motifs.
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Calcitriol / pharmacology*
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Chromobox Protein Homolog 5
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Chromosomal Proteins, Non-Histone / genetics*
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Chromosomal Proteins, Non-Histone / metabolism
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Estradiol / pharmacology*
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Gene Expression Regulation / drug effects*
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Humans
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RNA, Messenger / metabolism
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Receptors, Calcitriol / metabolism
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Regulatory Sequences, Nucleic Acid*
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription Factors / metabolism
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Transcription, Genetic*
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Tumor Cells, Cultured
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Vitamin D-Binding Protein / metabolism
Substances
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Chromosomal Proteins, Non-Histone
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RNA, Messenger
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Receptors, Calcitriol
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Transcription Factors
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Vitamin D-Binding Protein
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Chromobox Protein Homolog 5
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Estradiol
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Calcitriol