Cerebrospinal fluid and serum levels of cytokines and soluble tumor necrosis factor receptor in influenza virus-associated encephalopathy

Scand J Infect Dis. 2003;35(1):59-61. doi: 10.1080/0036554021000026986.

Abstract

This study determined the concentrations of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), soluble TNF receptor 1 (sTNFR1) and soluble E-selectin (sE-selectin) in cerebrospinal fluid (CSF) and serum from 15 children with influenza virus-associated encephalopathy to determine the role of cytokines in the pathogenesis. Cytokines and sTNFR1 were measured by enzyme-linked immunosorbent assay. The CSF IL-6, TNF-alpha and sTNFR1 concentrations were elevated in 9, 4 and 4 of 12 children, respectively. The serum concentrations of IL-6, TNF-alpha, sTNFR1 and sE-selectin were elevated in 10, 2, 5 and 7 of 13 children, respectively. Four children with elevated TNF-alpha and sTNFR1 levels in the CSF had neurological sequelae. The results suggested that cytokines not only in serum but also in CSF play a pivotal role in influenza virus-associated encephalopathy, and that the CSF TNF-alpha and sTNFR1 levels may be important for predicting neurological sequelae.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Biomarkers / analysis*
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Cohort Studies
  • Cytokines / analysis
  • Cytokines / metabolism*
  • E-Selectin / analysis
  • Encephalitis, Viral / blood
  • Encephalitis, Viral / cerebrospinal fluid
  • Encephalitis, Viral / complications
  • Encephalitis, Viral / diagnosis*
  • Female
  • Humans
  • Influenza, Human / complications
  • Influenza, Human / diagnosis*
  • Interleukin-6 / analysis
  • Male
  • Orthomyxoviridae / isolation & purification*
  • Prognosis
  • Receptors, Tumor Necrosis Factor / analysis
  • Receptors, Tumor Necrosis Factor / metabolism*
  • Sensitivity and Specificity
  • Solubility
  • Tumor Necrosis Factor-alpha / analysis

Substances

  • Biomarkers
  • Cytokines
  • E-Selectin
  • Interleukin-6
  • Receptors, Tumor Necrosis Factor
  • Tumor Necrosis Factor-alpha