Trehalose and glycogen accumulation is related to the duration of the G1 phase of Saccharomyces cerevisiae

FEMS Yeast Res. 2003 May;3(3):261-8. doi: 10.1111/j.1567-1364.2003.tb00168.x.


Several factors may control trehalose and glycogen synthesis, like the glucose flux, the growth rate, the intracellular glucose-6-phosphate level and the glucose concentration in the medium. Here, the possible relation of these putative inducers to reserve carbohydrate accumulation was studied under well-defined growth conditions in nitrogen-limited continuous cultures. We showed that the amounts of accumulated trehalose and glycogen were regulated by the growth rate imposed on the culture, whereas other implicated inducers did not exhibit a correlation with reserve carbohydrate accumulation. Trehalose accumulation was induced at a dilution rate (D)</=0.10 h(-1), whereas glycogen accumulation gradually increased at decreasing growth rates. The growth rate dependency of trehalose accumulation was supported by studies in cells overexpressing the G(1)-cyclin CLN3. The trehalose level appeared to be dependent on the duration of the G(1) phase, as trehalose was only accumulated at a G(1) phase duration of more than 5 h in both wild-type and CLN3-overexpressing cells. On the other hand, the glycogen level was reduced by CLN3 overexpression in a cell cycle-independent manner. A possible regulatory mechanism that links trehalose and glycogen accumulation to the growth rate is discussed.

MeSH terms

  • Carbon / metabolism
  • Cell Cycle / physiology
  • Culture Media
  • Cyclin G
  • Cyclins / genetics
  • Cyclins / metabolism
  • G1 Phase / physiology
  • Gene Expression Regulation, Fungal
  • Glycogen / biosynthesis*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Time Factors
  • Trehalose / biosynthesis*


  • Culture Media
  • Cyclin G
  • Cyclins
  • Carbon
  • Glycogen
  • Trehalose