Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses

Plant J. 2003 Apr;34(2):137-48. doi: 10.1046/j.1365-313x.2003.01708.x.


Many abiotic stress-inducible genes contain two cis-acting elements, namely a dehydration-responsive element (DRE; TACCGACAT) and an ABA-responsive element (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp promoter region (-174 to -55) of the Arabidopsis rd29A gene whose expression is induced by dehydration, high-salinity, low-temperature, and abscisic acid (ABA) treatments and whose 120 bp promoter region contains the DRE, DRE/CRT-core motif (A/GCCGAC), and ABRE sequences. Deletion and base substitution analyses of this region showed that the DRE-core motif functions as DRE and that the DRE/DRE-core motif could be a coupling element of ABRE. Gel mobility shift assays revealed that DRE-binding proteins (DREB1s/CBFs and DREB2s) bind to both DRE and the DRE-core motif and that ABRE-binding proteins (AREBs/ABFs) bind to ABRE in the 120 bp promoter region. In addition, transactivation experiments using Arabidopsis leaf protoplasts showed that DREBs and AREBs cumulatively transactivate the expression of a GUS reporter gene fused to the 120 bp promoter region of rd29A. These results indicate that DRE and ABRE are interdependent in the ABA-responsive expression of the rd29A gene in response to ABA in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology*
  • Arabidopsis / drug effects*
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / metabolism
  • Base Sequence
  • Dehydration / genetics*
  • Gene Expression Regulation, Plant / drug effects*
  • Genes, Reporter / genetics
  • Mutation / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Response Elements / genetics*
  • Sodium Chloride / pharmacology*
  • Transcription Factors / metabolism
  • Transcriptional Activation / drug effects


  • Arabidopsis Proteins
  • DREB1A protein, Arabidopsis
  • Transcription Factors
  • Sodium Chloride
  • Abscisic Acid