Analysis of two large functionally uncharacterized regions in the Methanopyrus kandleri AV19 genome

BMC Genomics. 2003 Apr 2;4(1):12. doi: 10.1186/1471-2164-4-12. Epub 2003 Apr 2.


Background: For most sequenced prokaryotic genomes, about a third of the protein coding genes annotated are "orphan proteins", that is, they lack homology to known proteins. These hypothetical genes are typically short and randomly scattered throughout the genome. This trend is seen for most of the bacterial and archaeal genomes published to date.

Results: In contrast we have found that a large fraction of the genes coding for such orphan proteins in the Methanopyrus kandleri AV19 genome occur within two large regions. These genes have no known homologs except from other M. kandleri genes. However, analysis of their lengths, codon usage, and Ribosomal Binding Site (RBS) sequences shows that they are most likely true protein coding genes and not random open reading frames.

Conclusions: Although these regions can be considered as candidates for massive lateral gene transfer, our bioinformatics analysis suggests that this is not the case. We predict many of the organism specific proteins to be transmembrane and belong to protein families that are non-randomly distributed between the regions. Consistent with this, we suggest that the two regions are most likely unrelated, and that they may be integrated plasmids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / genetics
  • Amino Acids / physiology
  • Archaeal Proteins / genetics
  • Archaeal Proteins / physiology
  • Base Composition
  • DNA, Archaeal / analysis
  • Genes, Archaeal* / physiology
  • Genome, Archaeal*
  • Multigene Family / genetics
  • Multigene Family / physiology
  • Open Reading Frames / genetics
  • Open Reading Frames / physiology
  • Predictive Value of Tests
  • Transcription Initiation Site


  • Amino Acids
  • Archaeal Proteins
  • DNA, Archaeal