The effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene

Toxicol Lett. 2003 Jun 5;143(1):37-44. doi: 10.1016/s0378-4274(03)00111-5.

Abstract

The interaction of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei (MN) and sister chromatid exchange (SCE) induced by benzo(a)pyrene (BP) in human lymphocytes was examined. A 60 Hz ELF-EMF of 0.8 mT field strength was applied either alone or with the tumor initiator, BP for 24 h. The frequencies of MN and SCE induced by BP increased in a dose-dependent manner. The co-exposure of cells to BP and 0.8 mT ELF-EMF for 24 h, followed by BP exposure for 48 h led to significant increases in the frequencies of MN and SCE compared to BP treatment for 72 h alone (P<0.05), but no significant difference was observed between field exposed and sham exposed control cells. The obtained results suggest that low density ELF-EMF could act as an enhancer of the initiation process of BP rather than as an initiator of mutagenic effects in human lymphocytes.

MeSH terms

  • Benzo(a)pyrene / pharmacology*
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Electromagnetic Fields*
  • Humans
  • Lymphocytes / drug effects*
  • Lymphocytes / radiation effects*
  • Micronuclei, Chromosome-Defective / drug effects*
  • Micronuclei, Chromosome-Defective / radiation effects*
  • Mutagens / pharmacology*
  • Sister Chromatid Exchange / drug effects*
  • Sister Chromatid Exchange / radiation effects*

Substances

  • Mutagens
  • Benzo(a)pyrene