Human chromosome 3: integration of 60 NotI clones into a physical and gene map

G E Sulimova; A S Kutsenko; E R Rakhmanaliev; I G Udina; A A Kompaniytsev; A I Protopopov; E V Moisjak; E A Klimov; O V Muravenko; A V Zelenin; E A Braga; V I Kashuba; E R Zabarovsky; L L Kisselev

doi:10.1159/000069814

Human chromosome 3: integration of 60 NotI clones into a physical and gene map

Cytogenet Genome Res. 2002;98(2-3):177-83. doi: 10.1159/000069814.

Authors

G E Sulimova¹, A S Kutsenko, E R Rakhmanaliev, I G Udina, A A Kompaniytsev, A I Protopopov, E V Moisjak, E A Klimov, O V Muravenko, A V Zelenin, E A Braga, V I Kashuba, E R Zabarovsky, L L Kisselev

Affiliation

¹ Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia. sge@vigg.ru

PMID: 12698000
DOI: 10.1159/000069814

Abstract

Sequence tagged sites generated for 60 NotI clones (NotI-STSs) from human chromosome 3-specific NotI-jumping and NotI-linking libraries were physically located using PCR screening of a radiation hybrid (RH) GeneBridge4 panel. The NotI map of chromosome 3 was generated using these RH-mapping data and those obtained earlier by FISH and sequencing of the corresponding NotI clones. The sequences of the NotI clones showed significant homologies with known genes and/or ESTs for 58 NotI-STSs (97%). These 58 NotI clones displayed 91-100% identity to 54 genes and 23 cDNA/EST clones. One known and two hypothetical protein-coding genes were localized for the first time and nine cDNA clones (unknown genes) were also carefully mapped only in this work. Three newly mapped genes are histone gene H1X (NR1-BK20C) and genes for hypothetical proteins THC1032178 and THC1024604 (NL1-243).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping*
Chromosomes, Human, Pair 3*
Cloning, Molecular
Deoxyribonucleases, Type II Site-Specific*
Humans
Radiation Hybrid Mapping*
Sequence Tagged Sites

Substances

Deoxyribonucleases, Type II Site-Specific
GCGGCCGC-specific type II deoxyribonucleases