Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions

FEMS Yeast Res. 2002 Dec;2(4):495-517. doi: 10.1111/j.1567-1364.2002.tb00117.x.


Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5' end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Basidiomycota / classification*
  • Basidiomycota / genetics
  • DNA, Fungal / genetics
  • DNA, Ribosomal / genetics*
  • DNA, Ribosomal Spacer / analysis*
  • DNA, Ribosomal Spacer / genetics
  • Genetic Variation*
  • Phylogeny
  • Sequence Analysis, DNA


  • DNA, Fungal
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer