Analysis of clonotype distribution and persistence for an influenza virus-specific CD8+ T cell response

Immunity. 2003 Apr;18(4):549-59. doi: 10.1016/s1074-7613(03)00087-6.


The spectrum of TCR V beta usage is compared for primary and recall CD8(+)D(b)PA(224)(+) T cell responses in mice with influenza pneumonia. Single-cell RT-PCR established that the same clonotypes were present in the lymphoid tissue and in the virus-infected lung. Longitudinal analysis indicated that the memory TCR repertoire reflects the primary response, with no decrease in diversity prior to (or after) secondary challenge. The re-engagement of memory T cells looked to be stochastic in this localized, transient infection. Analysis of clonotypes from the blood, spleen, regional lymph nodes, bone marrow, lung, and liver over a 200 day interval showed no evidence of selective localization or loss. The long-term distribution of memory T cells seemed to be essentially random.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology*
  • Epitopes, T-Lymphocyte
  • Female
  • Immunologic Memory
  • Influenza A virus / immunology*
  • Lung / immunology
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Antigen, T-Cell, alpha-beta / analysis
  • Spleen / immunology


  • Epitopes, T-Lymphocyte
  • Receptors, Antigen, T-Cell, alpha-beta