Reagentless detection of microorganisms by intrinsic fluorescence

Biosens Bioelectron. 2003 May;18(5-6):511-9. doi: 10.1016/s0956-5663(03)00008-3.


Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels ( approximately 20 cells/cm(2) or cm(3)) of microbes in seconds.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Bacteria / isolation & purification*
  • Colony Count, Microbial / instrumentation*
  • Colony Count, Microbial / methods*
  • Electronics
  • Equipment Design
  • Equipment Failure Analysis
  • Feasibility Studies
  • Fungi / isolation & purification*
  • Indicators and Reagents
  • Miniaturization
  • Pilot Projects
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Software
  • Spectrometry, Fluorescence / instrumentation*
  • Spectrometry, Fluorescence / methods*
  • Viruses / isolation & purification*


  • Indicators and Reagents