High frequency of DAP-kinase gene promoter methylation in colorectal cancer specimens and its identification in serum

Cancer Lett. 2003 May 8;194(1):99-105. doi: 10.1016/s0304-3835(03)00055-7.


Death-associated protein (DAP)-kinase is frequently inactivated by promoter methylation in human cancers. To understand the involvement of the DAP-kinase gene in colorectal cancer (CRC), we investigated the methylation of the DAP-kinasegene in primary CRC to define the frequency of this epigenetic aberration and the clinicopathological significance. For this reason, methylation-specific polymerase chain reaction (MSP) was used to detect DAP-kinase gene methylation in DNA from 122 cases of CRC and 18 paired serum samples. Methylation of the DAP-kinase gene was found in 67 of 122 (55%) cases of primary CRC. Study of the serum DNA from 14 patients exhibiting methylated DAP-kinase gene revealed aberrant methylation in three patients (21%). False positives were not obtained in any of the patients who did not exhibit methylation. No association was found between the promoter methylation of the DAP-kinase gene in primary CRC and gender, localization, tumor differentiation, invasion depth, regional lymph node involvement, or tumor stage. In conclusion, methylation of the DAP-kinase gene is common in CRC. The detection of the methylation of the DAP-kinase gene has a potential clinical application as a diagnostic tumor marker for CRC.

MeSH terms

  • Apoptosis Regulatory Proteins
  • Blotting, Western
  • Calcium-Calmodulin-Dependent Protein Kinases / blood
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Colorectal Neoplasms / blood
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism*
  • DNA / metabolism
  • DNA Methylation*
  • Death-Associated Protein Kinases
  • Female
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Sulfites / pharmacology


  • Apoptosis Regulatory Proteins
  • Sulfites
  • DNA
  • Death-Associated Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • sodium bisulfite