Functional analysis of CaRAP1, encoding the Repressor/activator protein 1 of Candida albicans

Gene. 2003 Mar 27;307:151-8. doi: 10.1016/s0378-1119(03)00456-6.

Abstract

The RAP1 gene (repressor/activator protein 1) encodes a transcription factor and telomere binding protein that is essential for viability in the budding yeast Saccharomyces cerevisiae. The genome sequence of the opportunistic fungal pathogen Candida albicans contains a RAP1 homologue. We generated C. albicans mutants in which both RAP1 alleles were deleted. The Deltarap1 mutants grew as well as the wild-type parental strain and formed normal germ tubes and hyphae in response to a variety of inducing conditions. However, under conditions that promote budding yeast growth in the wild-type strain, the Deltarap1 mutants formed both yeast and pseudohyphal cells. This phenotype was reverted upon reintroduction of a functional RAP1 copy. Our results demonstrate that RAP1 is a non-essential gene in C. albicans which is required to repress the formation of pseudohyphae under conditions favouring growth as budding yeast.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / genetics*
  • Candida albicans / growth & development
  • Cell Division / genetics
  • Cell Division / physiology
  • Fungal Proteins / genetics
  • Fungal Proteins / physiology*
  • Gene Deletion
  • Genes, Essential / genetics
  • Genetic Complementation Test
  • Mutation
  • Phenotype
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Telomere-Binding Proteins / genetics*
  • Telomere-Binding Proteins / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / physiology

Substances

  • Fungal Proteins
  • RAP1 protein, Candida albicans
  • Telomere-Binding Proteins
  • Transcription Factors