Inhibitory effect of menaquinone-7 (vitamin K2) on the bone-resorbing factors-induced bone resorption in elderly female rat femoral tissues in vitro

Mol Cell Biochem. 2003 Mar;245(1-2):115-20. doi: 10.1023/a:1022818111655.


The inhibitory effect of menaquinone-7 (MK-7; vitamin K2) on osteoclast-like cell formation and osteoclastic bone resorption in vitro is found (Mol Cell Biochem 228: 39-47, 2001). This study, furthermore, was undertaken to determine the effect of MK-7 on the bone-resorbing factor-induced bone resorption using the femoral-diaphyseal and -metaphyseal tissues obtained from elderly female rats in vitro. Femoral-diaphyseal and -metaphyseal tissues were cultured for 48 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained MK-7 (10(-7)-10(-5) M). The bone-resorbing factors, parathyroid hormone (1-34) (PTH; 10(-7) M) and prostaglandin E2 (PGE2; 10(-5) M), caused a significant decrease in calcium content in the diaphyseal and metaphyseal tissues. The PTH or PGE2-induced decrease in bone calcium content was completely inhibited in the presence of MK-7 (10(-7)-10(-5) M). In addition, MK-7 (10(-7)-10(-5) M) completely prevented the PTH (10(-7) M)- or PGE2 (10(-5) M)-induced increase in medium glucose consumption and lactic acid production by bone tissues. These results support the view that MK-7 has a direct inhibitory effect on the bone-resorbing factor-induced bone resorption in bone culture using female aged femoral tissues in vitro.

Publication types

  • Comparative Study

MeSH terms

  • Aging / drug effects*
  • Animals
  • Bone Resorption / drug therapy*
  • Bone and Bones / drug effects*
  • Bone and Bones / metabolism
  • Calcium / antagonists & inhibitors
  • Calcium / metabolism
  • Cells, Cultured
  • Culture Media
  • Dinoprostone / pharmacology
  • Female
  • Femur / drug effects
  • Femur / metabolism
  • Glucose / metabolism
  • Growth Inhibitors / pharmacology*
  • Lactic Acid / metabolism
  • Parathyroid Hormone / pharmacology
  • Peptide Fragments / pharmacology
  • Rats
  • Rats, Wistar
  • Vitamin K 2 / analogs & derivatives*
  • Vitamin K 2 / pharmacology*


  • Culture Media
  • Growth Inhibitors
  • Parathyroid Hormone
  • Peptide Fragments
  • Vitamin K 2
  • Lactic Acid
  • menaquinone 7
  • Glucose
  • Dinoprostone
  • Calcium