Loop-contraction mutagenesis of a type 1 copper site

J Am Chem Soc. 2003 Apr 30;125(17):4974-5. doi: 10.1021/ja0345827.

Abstract

Loop-contraction mutagenesis has been applied to the cupredoxin pseudoazurin to introduce the active-site loop of amicyanin. The mutation has a limited effect on the spectroscopic properties, and therefore structure, of the cupric protein. The loop contraction results in the increase of the pKa for the detachable His ligand of pseudoazurin by two pH units, similar to the value as found in amicyanin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcaligenes / chemistry
  • Azurin / analogs & derivatives*
  • Azurin / chemistry*
  • Bacterial Proteins / chemistry*
  • Binding Sites
  • Copper / chemistry*
  • Electron Spin Resonance Spectroscopy
  • Hydrogen-Ion Concentration
  • Kinetics
  • Metalloproteins / chemistry*
  • Mutagenesis
  • Paracoccus denitrificans / chemistry
  • Protein Conformation
  • Spectrophotometry, Ultraviolet
  • Structure-Activity Relationship

Substances

  • Bacterial Proteins
  • Metalloproteins
  • cupredoxin
  • mauC protein, Methylobacterium extorquens
  • pseudoazurin
  • Azurin
  • Copper