Jeotgalicoccus Halotolerans Gen. Nov., Sp. Nov. And Jeotgalicoccus Psychrophilus Sp. Nov., Isolated From the Traditional Korean Fermented Seafood Jeotgal

Int J Syst Evol Microbiol. 2003 Mar;53(Pt 2):595-602. doi: 10.1099/ijs.0.02132-0.

Abstract

Two Gram-positive, non-motile, non-spore-forming, halotolerant and moderately halophilic cocci (strains YKJ-101T and YKJ-115T) were isolated from the traditional Korean fermented seafood jeotgal, and were investigated using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rDNA sequences showed that strains YKJ-101T and YKJ-115T are most closely related to the cluster comprising two Salinicoccus species. The peptidoglycan type of the strains is A3alpha, based on L-Lys-Gly(3-4)-L-Ala(Gly), and the predominant menaquinone is MK-7. Strains YKJ-101T and YKJ-115T have cellular fatty acid profiles containing major amounts of saturated, unsaturated and branched fatty acids; the major fatty acids are anteiso-C15 : 0 and iso-C15 : 0. The cellular polar lipids are phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. Strains YKJ-101T and YKJ-115T have identical DNA G + C contents of 42 mol%. The 16S rDNA similarity between strains YKJ-101T and YKJ-115T is 98% and the mean level of DNA-DNA relatedness between the two strains is 13.4%. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, it is proposed that strains YKJ-101T and YKJ-115T should be placed in a new genus, Jeotgalicoccus gen. nov., as two distinct new species, for which the names Jeotgalicoccus halotolerans sp. nov. and Jeotgalicoccus psychrophilus sp. nov. are proposed. The type strains are YKJ-101T (=KCCM 41448T =JCM 11198T) and YKJ-115T (=KCCM 41449T =JCM 11199T), respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fatty Acids / analysis
  • Fermentation
  • Korea
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / analysis*
  • RNA, Ribosomal, 16S / genetics
  • Seafood / microbiology*
  • Sequence Analysis, DNA
  • Staphylococcaceae / classification*
  • Staphylococcaceae / genetics
  • Staphylococcaceae / isolation & purification
  • Staphylococcaceae / physiology*

Substances

  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AY028925
  • GENBANK/AY028926
  • GENBANK/AY028927