Effects of a new immunotherapeutic agent (CG5601) on endotoxin-induced uveitis

Ophthalmic Res. May-Jun 2003;35(3):143-9. doi: 10.1159/000070049.

Abstract

CG5601 is a novel immunomodulatory substance showing anti-inflammatory properties comparable to thalidomide. To investigate the anti-inflammatory effects of CG5601 in endotoxin-induced uveitis (EIU) and to evaluate its influence on leukocyte-endothelium interaction, the anterior chamber inflammatory reaction was assessed and intravital fluorescence microscopy was carried out at 2, 4, 8 and 24 h. Lewis rats received an intraperitoneal injection of CG5601 (200 mg/kg b.w.) at the time of lipopolysaccharide injection. At 8 and 24 h, CG5601 inhibited the cell migration and protein concentration in the aqueous humor compared to untreated EIU (p < 0.0001). There was no significant difference between nontreated animals and vehicle controls. The treatment of CG5601 reduced the number of rolling leukocytes. At early time points (2 and 4 h), inhibition of rolling leukocyte flux was significant (p < 0.005). The rise of serum TNF-alpha levels in EIU at 2 h was reduced. CG5601 exerts potent anti-inflammatory effects in EIU.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Aqueous Humor / metabolism
  • Disease Models, Animal
  • Drug Evaluation, Preclinical
  • Endotoxins
  • Female
  • Imidazoles / therapeutic use
  • Leukocyte Rolling / drug effects
  • Leukocytes / drug effects
  • Lipopolysaccharides / toxicity
  • Rats
  • Rats, Wistar
  • Spiro Compounds / therapeutic use
  • Thalidomide / analogs & derivatives
  • Thalidomide / therapeutic use
  • Thiazoles / therapeutic use
  • Time Factors
  • Tumor Necrosis Factor-alpha / metabolism
  • Uveitis / chemically induced
  • Uveitis / drug therapy*

Substances

  • Anti-Inflammatory Agents
  • CG5601
  • Endotoxins
  • Imidazoles
  • Lipopolysaccharides
  • Spiro Compounds
  • Thiazoles
  • Tumor Necrosis Factor-alpha
  • Thalidomide