Expression of HMGI(Y) associated with malignant phenotype of human gastric tissue

Histopathology. 2003 May;42(5):466-71. doi: 10.1046/j.1365-2559.2003.01618.x.


Aims: We evaluated the association between HMGI(Y) expression and the detection of malignant cells by simple reverse transcriptase-polymerase chain reaction (RT-PCR) and correlated the level of HMGI(Y) expression and the clinicopathological data in gastric cancer.

Methods and results: We analysed HMGI(Y) expression in 62 gastric cancer tissues and 28 normal gastric tissues by RT-PCR and immunohistochemical study. HMGI(Y) expression evidenced by RT-PCR was observed in 42 (67.7%) of 62 gastric cancer samples, whereas eight (28.6%) of 28 normal gastric tissues were positive (P = 0.001). In immunohistochemical staining for HMGI(Y), 48 (77.4%) of 62 gastric cancers were positive for HMGI(Y), whereas four (6.5%) of 62 normal gastric mucosae around the tumour were weakly positive, particularly in cells of some hyperplastic glands (P < 0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumour size, histological grade, invasion depth, or lymph node metastasis.

Conclusions: Our results suggest that HMGI(Y) expression could be associated with malignant phenotype in human gastric tissue, and that HMGI(Y) may contribute to gastric tumorigenesis.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / secondary
  • Gastric Mucosa / metabolism*
  • HMGA1a Protein / genetics
  • HMGA1a Protein / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Phenotype
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach / pathology
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology


  • RNA, Messenger
  • RNA, Neoplasm
  • HMGA1a Protein