The repair and recombination enzyme ERCC1 is not required for immunoglobulin class switching

DNA Repair (Amst). 2003 May 13;2(5):561-9. doi: 10.1016/s1568-7864(03)00021-1.

Abstract

Class switch recombination (CSR) is a programmed gene rearrangement in which a B cell which is producing IgM and IgD antibody develops into an IgG-, IgA- or IgE-expressing cell. This is achieved by recombination between switch regions located 5' of each of the immunoglobulin heavy chain constant regions, except Cdelta. The mechanism of CSR has not been resolved but it is thought to involve a double-strand break followed by end joining. It has previously been suggested that the nucleotide excision repair protein ERCC1 may be involved in CSR due to its known roles in removal of 3' single-stranded tails in various types of recombination. In this study, we examined class switching in cultured splenocytes from ERCC1-deficient mice and found no evidence of any deficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism
  • Base Sequence
  • DNA Repair*
  • DNA-Binding Proteins*
  • Endonucleases*
  • Flow Cytometry
  • Genotype
  • Immunoglobulin Class Switching*
  • Mice
  • Mice, Transgenic
  • Models, Genetic
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proteins / metabolism
  • Proteins / physiology*
  • Recombination, Genetic*
  • Spleen / cytology
  • Time Factors
  • Transgenes

Substances

  • DNA-Binding Proteins
  • Proteins
  • Endonucleases
  • Ercc1 protein, mouse