Phenylboronate-chitosan resins for adsorption of beta-amylase from soybean extracts

Appl Biochem Biotechnol. 2003 Spring:105 -108:829-42. doi: 10.1385/abab:108:1-3:829.


Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a beta-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of beta-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Boronic Acids*
  • Chitin* / analogs & derivatives*
  • Chitosan
  • Composite Resins / chemical synthesis*
  • Glycine max / enzymology*
  • Kinetics
  • beta-Amylase / chemistry
  • beta-Amylase / isolation & purification
  • beta-Amylase / metabolism*


  • Boronic Acids
  • Composite Resins
  • Chitin
  • Chitosan
  • beta-Amylase
  • benzeneboronic acid