GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines

J Hum Genet. 2003;48(6):331-335. doi: 10.1007/s10038-003-0026-2. Epub 2003 Apr 29.


Comparative genomic hybridization (CGH) analyses have detected gains of copy number on 13q, especially at 13q31-q32, in cell lines and primary cases of various types of lymphoma. Since amplification of chromosomal DNA is one of the mechanisms that can activate tumor-associated genes, and because 13q amplification had been reported in various other types of tumors as well, we attempted to define by fluorescence in situ hybridization (FISH) a common region at 13q31-q32 in which to explore genes that might be targets for the amplification events. Although the commonly amplified region we defined was relatively large (approximately 4 Mb), only one true gene, GPC5, was found there. GPC5 was over-expressed in lymphoma cell lines that had shown amplification, in comparison with those that had not. Our findings suggest that GPC5 is a likely target for amplification, and that over-expression of this gene may contribute to development and/or progression of lymphomas and other tumors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Chromosomes, Artificial, Bacterial / genetics
  • Chromosomes, Human, Pair 13 / genetics*
  • DNA, Neoplasm*
  • Extracellular Matrix Proteins
  • Gene Amplification*
  • Gene Dosage
  • Glypicans
  • Heparan Sulfate Proteoglycans / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lymphoma / genetics*
  • Lymphoma / metabolism
  • Lymphoma / pathology
  • RNA, Messenger / analysis


  • DNA, Neoplasm
  • Extracellular Matrix Proteins
  • GPC5 protein, human
  • Glypicans
  • Heparan Sulfate Proteoglycans
  • RNA, Messenger