[Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin]

Wei Sheng Yan Jiu. 2000 May 30;29(3):175-7.
[Article in Chinese]


To explore the carcinogenic effects of sterigmatocystin(ST), one of the predominant contaminating mycotoxins in high risk areas of cancer in China, mutation of tumor suppressor gene p53 and oncogene Ki-ras in human fetal lung cells in vitro induced by ST was studied using cell culture and silver-staining PCR-SSCP methods. The results showed that, within 22 weeks of ST treatment, no abnormalities were found for both p53 and Ki-ras gene in electrophoresis. Abnormal electrophoretic migration bands were seen at exon 8 of p53 gene and Ki-ras gene in ST-treated human lung fibroblast cells 22 weeks after ST treatment. Thus, the results further confirmed the carcinogenic effects of ST on human fetal lung tissue.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogens / toxicity
  • Fetus
  • Fibroblasts / cytology
  • Genes, p53*
  • Genes, ras*
  • Humans
  • Lung / cytology*
  • Lung / metabolism
  • Mutation*
  • Sterigmatocystin / toxicity*


  • Carcinogens
  • Sterigmatocystin