PKR Is Not Required for Interferon-Gamma Inhibition of VSV Replication in Neurons

Viral Immunol. 2003;16(1):87-96. doi: 10.1089/088282403763635474.

Abstract

In this report, the contribution of PKR to the IFN-gamma mediated inhibition of VSV replication in neurons was examined. IFN-gamma treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-gamma treatment. The phosphorylation state of PKR and its downstream target, eIF2alpha, were unaffected by either IFN-gamma or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-gamma to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-gamma mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Interferon-gamma / pharmacology*
  • Mice
  • Neurons / virology*
  • Tumor Cells, Cultured
  • Vesicular stomatitis Indiana virus / drug effects
  • Vesicular stomatitis Indiana virus / physiology*
  • Viral Proteins / metabolism
  • Virus Replication / drug effects*
  • eIF-2 Kinase / metabolism*

Substances

  • Viral Proteins
  • Interferon-gamma
  • eIF-2 Kinase