Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation

FEMS Microbiol Lett. 2003 Apr 25;221(2):249-55. doi: 10.1016/S0378-1097(03)00205-2.


A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.

MeSH terms

  • Base Sequence
  • DNA Primers
  • DNA, Fungal / analysis
  • Databases, Factual
  • Electrophoresis / methods
  • Genetic Variation
  • Genome, Fungal
  • Molecular Sequence Data
  • Mycological Typing Techniques*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / classification*
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Terminal Repeat Sequences / genetics*


  • DNA Primers
  • DNA, Fungal