Endotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206-1

FEMS Immunol Med Microbiol. 2003 May 15;36(1-2):1-7. doi: 10.1016/S0928-8244(03)00093-2.

Abstract

A synthetic lipid A of Helicobacter pylori strain 206-1 (compound HP206-1), which is similar to its natural lipid A, exhibited no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506). Furthermore, compound HP206-1 as well as its natural lipid A demonstrated no or very low mitogenic responses in murine spleen cell. On the other hand, compound HP206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, MKN-1, in comparison with compound 506. Furthermore, compound HP206-1 exhibited induction of tumor necrosis factor-alpha production in human peripheral blood mononuclear cells and the cytokine production was clearly inhibited by mouse anti-human Toll-like receptor (TLR) 4 monoclonal antibody HTA125. Our findings indicate that the chemically synthesized lipid A, mimicking the natural lipid A portion of lipopolysaccharide from H. pylori strain 206-1, has a low endotoxic potency and immunobiological activities, and is recognized by TLR4.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Helicobacter pylori / immunology*
  • Helicobacter pylori / metabolism
  • Humans
  • Interleukin-8 / immunology
  • Interleukin-8 / metabolism
  • Lethal Dose 50
  • Lipid A / chemical synthesis
  • Lipid A / immunology
  • Lipid A / metabolism
  • Lipid A / toxicity*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Rabbits
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Interleukin-8
  • Lipid A
  • Tumor Necrosis Factor-alpha