Abstract
The lifespan of human foreskin fibroblasts (HFF5), cultured under standard in vitro conditions (including ambient atmospheric oxygen tension), was extended slightly by expression of exogenous mortalin (mot-2)/mthsp70/Grp75, but not by the catalytic subunit of telomerase, hTERT. Together, mot-2 and hTERT permitted bypass of senescence, a substantial extension of lifespan, and possibly immortalization. This is the first demonstration that mot-2 and telomerase can cooperate in the immortalization process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Division / physiology
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Cells, Cultured
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Cellular Senescence / physiology*
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Cyclin-Dependent Kinase Inhibitor p16 / metabolism
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DNA Fingerprinting
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DNA-Binding Proteins
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Fibroblasts / cytology
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Fibroblasts / physiology*
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HSP70 Heat-Shock Proteins / genetics
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HSP70 Heat-Shock Proteins / metabolism*
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Humans
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Mitochondrial Proteins
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Telomerase / genetics
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Telomerase / metabolism*
Substances
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Cyclin-Dependent Kinase Inhibitor p16
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DNA-Binding Proteins
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HSP70 Heat-Shock Proteins
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HSPA9 protein, human
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Mitochondrial Proteins
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mortalin
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Telomerase