Food-borne radiolytic compounds (2-alkylcyclobutanones)may promote experimental colon carcinogenesis

Nutr Cancer. 2002;44(2):189-91. doi: 10.1207/S15327914NC4402_11.

Abstract

Food irradiation is acknowledged as a safe process to improve food quality by reducing microbial contamination. Information on the toxicological potential of 2-alkylcyclobutanones (2-ACBs), radiolytic derivatives of triglycerides found exclusively in irradiated food, is scarce. Wistar rats received daily a solution of highly pure 2-tetradecylcyclobutanone (2-tDCB) or 2-(tetradec-5-enyl)-cyclobutanone (2-tDeCB) at a concentration of 0.005% in 1% ethanol as drinking fluid, while control animals received 1% ethanol. All animals received a single intraperitoneal injection of the chemical carcinogen azoxymethane (AOM) at Weeks 3 and 4. At 3 mo after AOM injection, no significant changes were observed in the total number of preneoplastic lesions in the colon of AOM controls and 2-ACB-treated animals. After 6 mo, the total number of tumors in the colon was threefold higher in the 2-ACB-treated animals than in the AOM controls. The colon of four of six AOM control rats exhibited only one small tumor ( &6 mm3). Multiple tumors were observed in four and three of six animals treated with 2-tDCB or 2-tDeCB, respectively. Medium (6 < S < 25 mm3) and larger (>25 mm3) tumors were detected only in 2-ACB-treated animals. This is the first demonstration that a compound found exclusively in irradiated dietary fats may promote colon carcinogenesis in animals treated with a chemical carcinogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Azoxymethane / administration & dosage
  • Azoxymethane / adverse effects
  • Carcinogens / administration & dosage
  • Carcinogens / adverse effects
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / etiology*
  • Cyclobutanes / adverse effects*
  • Disease Models, Animal
  • Food Irradiation / adverse effects*
  • Male
  • Neoplasms, Experimental / chemically induced
  • Rats
  • Rats, Wistar

Substances

  • Carcinogens
  • Cyclobutanes
  • Azoxymethane