Abstract
A strain of Saccharomyces cerevisiae, defective in small subunit ribosomal RNA processing, has a mutation in YOR145c ORF that converts Gly235 to Asp. Yor145c is a nucleolar protein required for cell viability and has been reported recently to be present in 90S pre-ribosomal particles. The Gly235Asp mutation in YOR145c is found in a KH-type RNA-binding domain and causes a marked deficiency in 18S rRNA production. Detailed studies by northern blotting and primer extension analyses show that the mutant strain impairs the early pre-rRNA processing cleavage essentially at sites A1 and A2, leading to accumulation of a 22S dead-end processing product that is found in only a few rRNA processing mutants. Furthermore, U3, U14, snR10 and snR30 snoRNAs, involved in early pre-rRNA cleavages, are not destabilized by the YOR145c mutation. As the protein encoded by YOR145c is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as RRP20.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Conserved Sequence / genetics
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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Genetic Complementation Test
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Humans
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Molecular Sequence Data
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Mutation
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Nuclear Proteins / metabolism*
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RNA Precursors / genetics
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RNA Precursors / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Ribosomal / genetics
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RNA, Ribosomal / metabolism
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RNA, Ribosomal, 18S / genetics
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RNA, Ribosomal, 18S / metabolism*
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism*
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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DNA, Fungal
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Nuclear Proteins
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PNO1 protein, S cerevisiae
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RNA Precursors
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RNA, Ribosomal
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RNA, Ribosomal, 18S
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RNA-Binding Proteins
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins