Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein

Cell Res. 2003 Apr;13(2):83-91. doi: 10.1038/


We have identified IC53-2, a human homologue of the rat C53 gene from a human placenta cDNA library (GeneBank Accession No.AF217982). IC53-2 can bind to the CDK5 activator p35 by in vitro association assay. IC53-2 is mapped to human chromosome 17q21.31. The IC53-2 transcript is highly expressed in kidney, liver, skeletal muscle and placenta. It is abundantly expressed in SMMC-7721, C-33A, 3AO, A431 and MCF-7 cancer cell lines by RT-PCR assay. Stable transfection of IC53-2 cDNA into the hepatocellular carcinoma SMMC-7721 cell remarkably stimulates its growth in vitro. The above results indicate that IC53-2 is a novel human gene, which may be involved in the regulation of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / metabolism
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification*
  • Cell Cycle Proteins
  • Cell Division / genetics*
  • Cell Transformation, Neoplastic / genetics*
  • Cell Transformation, Neoplastic / metabolism
  • Chromosomes, Human, Pair 17
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 5
  • Cyclin-Dependent Kinases / genetics*
  • Gene Expression Regulation, Neoplastic / genetics
  • HeLa Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / isolation & purification*
  • Protein Binding / genetics
  • Protein Isoforms / genetics
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Tumor Suppressor Proteins


  • CDK5RAP3 protein, human
  • Carrier Proteins
  • Cell Cycle Proteins
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Protein Isoforms
  • Tumor Suppressor Proteins
  • neuronal Cdk5 activator (p25-p35)
  • Cyclin-Dependent Kinase 5
  • CDK5 protein, human
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/AF217982