Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells

Dev Cell. 2003 May;4(5):753-9. doi: 10.1016/s1534-5807(03)00122-9.


The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells. Inhibition of their function by overexpression of nonmembrane-anchored mutants causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission but not further upstream events, such as furrowing, are affected. These results indicate that successful completion of cytokinesis requires a SNARE-mediated membrane fusion event and that this requirement is distinct from exocytic events that may be involved in prior ingression of the plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism*
  • CHO Cells
  • Cell Division
  • Cell Line
  • Cricetinae
  • Dogs
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • R-SNARE Proteins
  • Rats
  • Syntaxin 1
  • Time Factors


  • Antigens, Surface
  • Membrane Proteins
  • Nerve Tissue Proteins
  • R-SNARE Proteins
  • Syntaxin 1
  • Vamp8 protein, rat