Failure to detect Chlamydia pneumoniae by cell culture and polymerase chain reaction in major arteries of 93 patients with atherosclerosis

Eur J Clin Microbiol Infect Dis. 2003 May;22(5):300-2. doi: 10.1007/s10096-003-0912-8. Epub 2003 May 9.

Abstract

To detect Chlamydia pneumoniae in punch specimens of the aortic wall of 61 patients undergoing coronary-aortic bypass graft, and carotid atheromas of 32 patients undergoing carotid endarterectomy, cell culture (HEp-2 cells) and two polymerase chain reaction assays in two different laboratories were used. All cultures and polymerase chain reaction tests for Chlamydia pneumoniae were negative. Further studies are required to explore the complex relationship between Chlamydia pneumoniae and atherosclerosis.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Carotid Stenosis / microbiology*
  • Carotid Stenosis / surgery
  • Cells, Cultured
  • Chlamydia Infections / diagnosis
  • Chlamydophila pneumoniae / isolation & purification*
  • Cohort Studies
  • Coronary Artery Bypass / methods
  • Coronary Artery Disease / microbiology*
  • Coronary Artery Disease / surgery
  • DNA, Bacterial / analysis
  • Endarterectomy, Carotid / methods
  • False Negative Reactions
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Preoperative Care
  • Prospective Studies
  • Risk Assessment
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial