Detection of a mammalian-like group A rotavirus in diarrhoeic chicken

Vet Microbiol. 2003 Jun 24;94(1):13-8. doi: 10.1016/s0378-1135(03)00079-8.

Abstract

The present investigation describes detection of a mammalian-like electropherogroup A rotavirus in chicken with diarrhoea. This also records the first detection of a rotavirus in an avian species from India. During the investigation 75 diarrhoeic faecal samples collected from adult chicken were screened for the presence of group A rotavirus antigen by sandwich ELISA. All three samples positive for rotavirus antigen revealed 11 bands of RNA in polyacrylamide gel electrophoresis (PAGE). In contrast to avian group A rotavirus, segment 5 was found to migrate closer to 6 as is the case with mammalian group A rotaviruses. Segments 7, 8 and 9 were found to migrate as a tight triplet, which is characteristic of group A rotavirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / metabolism
  • Chickens
  • Diarrhea / veterinary*
  • Diarrhea / virology
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Feces / virology
  • India
  • Poultry Diseases / virology*
  • RNA, Viral / chemistry
  • RNA, Viral / genetics
  • Rotavirus / classification
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / veterinary*
  • Rotavirus Infections / virology

Substances

  • Antigens, Viral
  • RNA, Viral