Abstract
Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Carrier Proteins / genetics*
-
Carrier Proteins / metabolism
-
Cysteine Endopeptidases / genetics
-
Cysteine Endopeptidases / metabolism*
-
DNA, Complementary / chemistry
-
DNA, Complementary / genetics
-
Molecular Sequence Data
-
Plant Proteins / genetics*
-
Plant Proteins / metabolism
-
Protein Binding
-
Saccharomyces cerevisiae / genetics
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Solanum tuberosum / genetics*
-
Solanum tuberosum / metabolism
-
Two-Hybrid System Techniques
-
Viral Proteins / genetics
-
Viral Proteins / metabolism*
-
Zinc Fingers / genetics*
Substances
-
Carrier Proteins
-
DNA, Complementary
-
Plant Proteins
-
Viral Proteins
-
Cysteine Endopeptidases
-
HC-Pro protein, potyvirus
Associated data
-
GENBANK/AJ507316
-
GENBANK/AJ507317