Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro

Mol Plant Microbe Interact. 2003 May;16(5):405-10. doi: 10.1094/MPMI.2003.16.5.405.


Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism*
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Protein Binding
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Solanum tuberosum / genetics*
  • Solanum tuberosum / metabolism
  • Two-Hybrid System Techniques
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*
  • Zinc Fingers / genetics*


  • Carrier Proteins
  • DNA, Complementary
  • Plant Proteins
  • Viral Proteins
  • Cysteine Endopeptidases
  • HC-Pro protein, potyvirus

Associated data

  • GENBANK/AJ507316
  • GENBANK/AJ507317