A SWI/SNF-like factor from chicken liver that disrupts nucleosomes and transfers histone octamers in cis and trans

Arch Biochem Biophys. 2003 Jun 1;414(1):24-33. doi: 10.1016/s0003-9861(03)00175-9.


ATP-dependent chromatin remodeling factors have been implicated in nuclear processes involving DNA. Here we report partial purification and characterization of an ATP-dependent chromatin remodeling activity from chicken liver. Nuclear extract from chicken liver was fractionated chromatographically to enrich proteins immunoreacting to antibodies against components of human SWI/SNF, namely BRG1, BAF170, BAF155, and BAF57. Immunoreactivity to these antibodies elutes with a mass of about 2MDa on Sepharose CL-6B gel filtration, suggesting that they constitute a SWI/SNF-like complex (SLC). The SLC displays three chromatin-remodeling activities, viz. nucleosome disruption, octamer transfer, and nucleosome sliding (octamer transfer in cis). We further show that components of SLC, as revealed by immunoreactivity to the above antibodies, display a dynamic nucleocytoplasmic distribution and colocalize with RNA polymerase II in the liver nuclei. This report contributes to the understanding of phylogenetic generality of chromatin remodeling factors in eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Chickens
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Chromosomal Proteins, Non-Histone / isolation & purification*
  • DNA Damage
  • Histones / chemistry*
  • In Vitro Techniques
  • Liver / chemistry*
  • Liver / metabolism
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / metabolism
  • Nucleosomes / chemistry*
  • Stereoisomerism
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism


  • Chromosomal Proteins, Non-Histone
  • Histones
  • Nuclear Proteins
  • Nucleosomes
  • Transcription Factors
  • Adenosine Triphosphate