A clinicopathological review of 34 cases of inflammatory breast disease showing an association between corynebacteria infection and granulomatous mastitis

Pathology. 2003 Apr;35(2):109-19.


Aim: Granulomatous mastitis is a rare condition of unknown aetiology. The great majority of cases has not been associated with bacterial pathogens if women with mammary tuberculosis are excluded. We noted that some women in Auckland with a histological diagnosis of granulomatous mastitis had both microbiological and histological evidence of corynebacteria infection and aimed to study this further.

Methods: Thirty-four women were reviewed who presented with inflammatory breast disease and had microbiological specimens from which corynebacteria were isolated and/or histological specimens containing coryneform bacteria. These 34 cases were compared with 28 controls with similar histology but no evidence of corynebacteria infection.

Results: Twenty-seven (79%) of the cases and 21 (75%) of the controls had histological and/or cytological evidence of suppurative granulomas. Fourteen of the 34 cases also had Gram-positive bacilli (GPB), recognisable as coryneform bacteria, in histological sections. In all cases the bacilli were confined to empty spaces, consistent with dissolved lipid, and were surrounded by neutrophils and, frequently, suppurative granulomas. Corynebacterium species were isolated from 52 of 116 microbiological specimens taken from the 34 cases. Forty of these 52 cultures were pure. Twenty-four of the cultures were further classified biochemically and using 16S rRNA gene sequencing. Twenty of the 24 were lipophilic Corynebacterium species and 14 were identified as Corynebacterium kroppenstedtii. The cases were more likely to present with fever or neutrophilia and more often formed sinuses than the controls but other clinical features were similar. Maori and Pacific Islanders accounted for 77% of the women across both groups.

Conclusion: We suggest granulomatous mastitis can be associated with corynebacteria infection, particularly infection by C. kroppenstedtii. The significance of this finding, which has previously been described in only a single case report, is discussed.

MeSH terms

  • Adult
  • Corynebacterium / classification
  • Corynebacterium / genetics
  • Corynebacterium / isolation & purification*
  • Corynebacterium Infections / complications
  • Corynebacterium Infections / ethnology
  • Corynebacterium Infections / pathology*
  • Female
  • Genes, Bacterial
  • Granuloma / ethnology
  • Granuloma / microbiology
  • Granuloma / pathology*
  • Humans
  • Mastitis / ethnology
  • Mastitis / microbiology
  • Mastitis / pathology*
  • Native Hawaiian or Other Pacific Islander
  • New Zealand / epidemiology
  • RNA, Bacterial / analysis
  • RNA, Ribosomal, 16S / analysis


  • RNA, Bacterial
  • RNA, Ribosomal, 16S