Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity

J Mol Med (Berl). 2003 Jul;81(7):428-34. doi: 10.1007/s00109-002-0409-4. Epub 2003 May 16.


Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (> or =27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m(2) in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / metabolism*
  • Adiponectin
  • Adult
  • Alleles
  • Animals
  • Blood Glucose / metabolism
  • Body Mass Index*
  • Female
  • Gene Expression
  • Glucose Tolerance Test
  • Humans
  • Insulin / pharmacology
  • Insulin / physiology
  • Insulin Resistance
  • Intercellular Signaling Peptides and Proteins*
  • Male
  • Mice
  • Middle Aged
  • Obesity / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Protein Biosynthesis*
  • Proteins / genetics*
  • Proteins / metabolism


  • Adiponectin
  • Blood Glucose
  • Insulin
  • Intercellular Signaling Peptides and Proteins
  • Proteins