CD4(+)CD3(-) accessory cells costimulate primed CD4 T cells through OX40 and CD30 at sites where T cells collaborate with B cells

Immunity. 2003 May;18(5):643-54. doi: 10.1016/s1074-7613(03)00110-9.


In this report we identify an accessory cell that interacts with primed and memory T cells at sites where they collaborate with B cells. These cells are distinguished from conventional dendritic cells by their lack of response to Flt3 ligand and their inability to process antigen. Unlike dendritic cells, the CD4(+)CD3(-) cells have little CD80 or CD86 expression but do express high levels of the TNF ligands, OX40 ligand and CD30 ligand. We show that Th2-primed cells express the receptors for these TNF ligands and preferentially survive when cocultured with these cells. Furthermore, we show that the preferential survival of OX40(+) T cells and support of memory T cell help for B cells are linked to their association with CD4(+)CD3(-) cells in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • B-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / immunology*
  • Coculture Techniques
  • Ki-1 Antigen / immunology*
  • Lymphocyte Cooperation*
  • Membrane Glycoproteins / immunology*
  • Mice
  • Microscopy, Confocal
  • OX40 Ligand
  • Signal Transduction / immunology
  • Spleen / cytology
  • Spleen / immunology
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Tumor Necrosis Factors


  • Ki-1 Antigen
  • Membrane Glycoproteins
  • OX40 Ligand
  • Tnfsf4 protein, mouse
  • Tumor Necrosis Factors