Identification and characterisation of human aldose 1-epimerase

FEBS Lett. 2003 May 22;543(1-3):21-4. doi: 10.1016/s0014-5793(03)00364-8.


Aldose 1-epimerase or mutarotase (EC is a key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha- and beta-anomers of hexose sugars such as glucose and galactose. We identified an open reading frame in the human genome (BC014916) which has high sequence similarity to previously identified bacterial aldose 1-epimerases. This sequence was cloned into a bacterial expression vector, and expressed and purified from this source. Enzyme assays show that the protein has aldose 1-epimerase activity and exhibits a preference for galactose over glucose. Site-directed mutagenesis confirmed the involvement of three residues involved in catalysis and substrate binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbohydrate Epimerases / chemistry*
  • Carbohydrate Epimerases / genetics
  • Carbohydrate Epimerases / metabolism*
  • Catalytic Domain
  • Cloning, Molecular
  • Galactose / metabolism
  • Glucose / metabolism
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Substrate Specificity


  • Recombinant Fusion Proteins
  • Carbohydrate Epimerases
  • aldose 1-epimerase
  • Glucose
  • Galactose