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. 2003 May 22;543(1-3):140-3.
doi: 10.1016/s0014-5793(03)00427-7.

Regulatory Mechanism of Cordyceps Sinensis Mycelium on Mouse Leydig Cell Steroidogenesis

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Regulatory Mechanism of Cordyceps Sinensis Mycelium on Mouse Leydig Cell Steroidogenesis

Chih-Chao Hsu et al. FEBS Lett. .
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Abstract

We demonstrate the mechanism by which Cordyceps sinensis (CS) mycelium regulates Leydig cell steroidogenesis. Mouse Leydig cells were treated with forskolin, H89, phorbol 12-myristate 13-acetate, staurosporine, or steroidogenic enzyme precursors with or without 3 mg/ml CS; then testosterone production was determined. H89, but not phorbol 12-myristate 13-acetate or staurosporine, decreased CS-treated Leydig cell steroidogenesis. CS inhibited Leydig cell steroidogenesis by suppressing the activity of P450scc enzyme, but not 3beta-hydroxysteroid dehydrogenase, 17alpha-hydroxylase, 20alpha-hydroxylase, or 17beta-hydroxysteroid dehydrogenase enzymes. Thus, CS activated the cAMP-protein kinase A signal pathway, but not protein kinase C, and attenuated P45scc enzyme activity to reduce human chorionic gonadotropin-stimulated steroidogenesis in purified mouse Leydig cells.

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