Regulatory mechanism of Cordyceps sinensis mycelium on mouse Leydig cell steroidogenesis

FEBS Lett. 2003 May 22;543(1-3):140-3. doi: 10.1016/s0014-5793(03)00427-7.

Abstract

We demonstrate the mechanism by which Cordyceps sinensis (CS) mycelium regulates Leydig cell steroidogenesis. Mouse Leydig cells were treated with forskolin, H89, phorbol 12-myristate 13-acetate, staurosporine, or steroidogenic enzyme precursors with or without 3 mg/ml CS; then testosterone production was determined. H89, but not phorbol 12-myristate 13-acetate or staurosporine, decreased CS-treated Leydig cell steroidogenesis. CS inhibited Leydig cell steroidogenesis by suppressing the activity of P450scc enzyme, but not 3beta-hydroxysteroid dehydrogenase, 17alpha-hydroxylase, 20alpha-hydroxylase, or 17beta-hydroxysteroid dehydrogenase enzymes. Thus, CS activated the cAMP-protein kinase A signal pathway, but not protein kinase C, and attenuated P45scc enzyme activity to reduce human chorionic gonadotropin-stimulated steroidogenesis in purified mouse Leydig cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstenedione / metabolism
  • Animals
  • Cells, Cultured
  • Colforsin / pharmacology
  • Cordyceps / physiology*
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Enzyme Inhibitors / pharmacology
  • Hydroxycholesterols / pharmacology
  • Isoquinolines / pharmacology
  • Leydig Cells / drug effects
  • Leydig Cells / enzymology
  • Leydig Cells / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mycelium / physiology
  • Progestins / metabolism
  • Protein Kinase C / antagonists & inhibitors
  • Staurosporine / pharmacology
  • Sulfonamides*
  • Testosterone / biosynthesis*
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Enzyme Inhibitors
  • Hydroxycholesterols
  • Isoquinolines
  • Progestins
  • Sulfonamides
  • 22-hydroxycholesterol
  • Colforsin
  • Testosterone
  • Androstenedione
  • Cyclic AMP-Dependent Protein Kinases
  • Protein Kinase C
  • Staurosporine
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • Tetradecanoylphorbol Acetate