Cloning genomic INGAP: a Reg-related family member with distinct transcriptional regulation sites

Biochim Biophys Acta. 2003 May 20;1638(1):83-9. doi: 10.1016/s0925-4439(03)00042-5.


The protein product of hamster islet neogenesis-associated protein (INGAP) cDNA induces new pancreatic islet development. Manipulation of this process provides a new therapeutic strategy for the treatment of diabetes. As regulators of INGAP gene expression are unknown over 6 kb of hamster genomic INGAP has been cloned. Sequence analysis has identified a 3 kb 5-prime region with core promoter elements that is rich in transcription factor binding sites and six exons for the coding region. Analysis of promoter activity reveals stimulus-responsive DNA elements which have been identified though deletion analysis. Comparison of transcription factor binding sites in INGAP to the related gene RegIIIdelta exposes potential sites for differential gene regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Neoplasm*
  • Base Sequence
  • Binding Sites / genetics
  • Biomarkers, Tumor*
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • DNA / genetics
  • Diabetes Mellitus / therapy
  • Gene Expression Regulation
  • Genes, Regulator
  • Genes, Reporter
  • Humans
  • Introns
  • Islets of Langerhans / growth & development
  • Islets of Langerhans / metabolism
  • Lectins, C-Type*
  • Molecular Sequence Data
  • Pancreatitis-Associated Proteins
  • Promoter Regions, Genetic
  • Proteins / genetics*
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Transfection


  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Lectins, C-Type
  • Pancreatitis-Associated Proteins
  • Proteins
  • REG3A protein, human
  • Reg3b protein, mouse
  • RegIII protein, mouse
  • DNA

Associated data

  • GENBANK/AY184211