Elevated Levels of interleukin-18 and Tumor Necrosis Factor-Alpha in Serum of Patients With Type 2 Diabetes Mellitus: Relationship With Diabetic Nephropathy

Metabolism. 2003 May;52(5):605-8. doi: 10.1053/meta.2003.50096.

Abstract

To compare levels of interleukin (IL)-18, tumor necrosis factor-alpha (TNF-alpha), and IL-6 in serum, we studied 151 type 2 diabetes mellitus patients with various degrees of nephropathy, as well as 80 healthy volunteers. IL-18, TNF-alpha, and IL-6 in serum were measured using an enzyme-linked immunosorbent assay (ELISA) with the respective mouse monoclonal antibodies. Significant differences in serum levels of IL-18 and TNF-alpha were observed between the patients and control subjects (IL-18, 278.0 +/- 11.9 pg/mL v 172.8 +/- 7.7 pg/mL, P <.0001; TNF-alpha, 2.41 +/- 0.18 pg/mL v 0.46 +/- 0.18 pg/mL, P <.0001), whereas that of IL-6 was not different between the two groups (0.73 +/- 0.10 pg/mL v 0.65 +/- 0.08 pg/mL, difference not significant [NS]), although patients with nephropathy showed higher levels. In addition, IL-18 levels were increased in diabetic patients with the development of urinary albumin excretion, with the highest found in those with microalbuminuria (<30 micro g/mg creatinine, 252.7 +/- 16.4 pg/mL; 30 to >300 micro g/mg creatinine, 352.7 +/- 35.2 pg/mL; >>300 micro g/mg creatinine, 350.0 +/- 16.0 pg/mL). Similarly, TNF-alpha and IL-6 in diabetic patients with microalbuminuria or clinical albuminuria were significantly increased as compared with those without albuminuria (TNF-alpha, 3.20 +/- 0.41 pg/mL v 1.94 +/- 0.18 pg/mL; IL-6, 1.64 +/- 1.11 pg/mL v 0.51 +/- 0.05 pg/mL, P <.05, respectively). These results suggest that serum levels of IL-18, TNF-alpha, and IL-6 may have some etiopathogenic roles in diabetic nephropathy.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Albuminuria / metabolism
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / pathology*
  • Diabetic Nephropathies / blood*
  • Diabetic Nephropathies / pathology*
  • Diabetic Nephropathies / urine
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Interleukin-18 / blood*
  • Interleukin-6 / blood
  • Lipids / blood
  • Male
  • Middle Aged
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Interleukin-18
  • Interleukin-6
  • Lipids
  • Tumor Necrosis Factor-alpha