Haemopoietic progenitor cells in prenatal congenitally anaemic 'flexed-tailed' (f/f) mice

Br J Haematol. 1976 Jul;33(3):387-94. doi: 10.1111/j.1365-2141.1976.tb03555.x.


The incidences of erythroid colony forming cells (CFUe) and granulocyte-macrophage colony forming cells (CFUc) have been measured in 11-18 d prenatal livers of mice of genotype f/f and nearly congenic +/+ controls. In normal fetal livers numbers of CFUe (cells able to form colonies of 16 or more cells after 72 h in vitro) rise to a maximum on day 14 of gestation and represent c 1% of total fetal liver cells. In f/f fetal livers, peak values for numbers and proportions of CFUe are 50% of normal. The f/f lesion does not reduce the numbers of CFUc in fetal liver. Since this deficiency in CFUe parallels deficiencies of similar magnitude in spleen-colony forming units (CFUs) and erythroblasts in the liver, and erythrocytes in the blood, of f/f fetuses it is concluded that the f/f lesion is expressed at an early stage of haemopoietic development in prenatal life. The possibility that restricted haem synthesis is the primary effect of the f/f genotype and responsible for disturbances of both haemopoietic cellular proliferation and haemoglobin synthesis is examined.

MeSH terms

  • Anemia / congenital*
  • Animals
  • Bone Marrow Cells
  • Erythrocytes / cytology
  • Erythropoietin / pharmacology
  • Female
  • Fetus / cytology
  • Granulocytes / cytology
  • Hematopoiesis*
  • Liver / embryology
  • Macrophages / cytology
  • Mice
  • Pregnancy


  • Erythropoietin