Expression of Na,K-ATPase in P. pastoris: Fe2+-catalyzed cleavage of the recombinant enzyme

Ann N Y Acad Sci. 2003 Apr:986:247-8. doi: 10.1111/j.1749-6632.2003.tb07173.x.

Authors

David Strugatsky¹, Rivka Goldshleger, Eitan Bibi, Steven J D Karlish

Affiliation

¹ Department of Biological Chemistry, Weizmann Institute of Science, Rehovoth 76100, Israel.

PMID: 12763809
DOI: 10.1111/j.1749-6632.2003.tb07173.x

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acid Substitution
Animals
Catalysis
Iron / pharmacology*
Kinetics
Mutagenesis, Site-Directed
Pichia / enzymology*
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Sodium-Potassium-Exchanging ATPase / chemistry
Sodium-Potassium-Exchanging ATPase / metabolism*
Swine

Substances

Recombinant Proteins
Iron
Sodium-Potassium-Exchanging ATPase