The TRPV6 gene, cDNA and protein

Cell Calcium. May-Jun 2003;33(5-6):509-18. doi: 10.1016/s0143-4160(03)00066-6.

Abstract

The mouse TRPV6 gene is localized on chromosome 6 and extends over 15.66kb. The encoded protein comprises 727 amino acid residues with a calculated relative molecular mass of 83,210Da. TRPV6 is glycosylated and both variants, the glycosylated and the de-glycosylated proteins, are recognized by various polyclonal and monoclonal antibodies, which were raised against TRPV6. Like human TRPV6, mouse TRPV6 binds calmodulin in the presence, but not in the absence of Ca2+. TRPV6 is abundantly expressed in mouse pancreas and placenta, and to a much lesser extend in mouse stomach and kidney. No transcript expression was detected in poly(A)+RNA isolated from heart, brain, intestine, esophagus or aortic endothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Calcium / metabolism*
  • Calcium Channels / genetics*
  • Calcium Channels / immunology
  • Calcium Channels / metabolism*
  • Calmodulin / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / chemistry*
  • DNA, Complementary / genetics
  • Glycosylation
  • Humans
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments / immunology
  • Placenta / chemistry*
  • Poly A / genetics
  • Rabbits
  • Sequence Homology, Amino Acid
  • TRPV Cation Channels
  • Tissue Distribution

Substances

  • Calcium Channels
  • Calmodulin
  • DNA, Complementary
  • Peptide Fragments
  • TRPV Cation Channels
  • TRPV6 channel
  • Poly A
  • Calcium