Abstract
Dof is a large molecule essential for signal transduction by the two FGF receptors in Drosophila. It contains two ankyrin repeats and a coiled-coil region, but has no other recognisable structural motif. Dof shares these features with its closest vertebrate relatives, the B-cell signalling molecules BCAP and BANK. In addition, this family of proteins shares a region of homology upstream of the ankyrin repeats, which we call the Dof/BCAP/BANK (DBB) motif. We have identified 44 proteins that interact with Dof in a yeast two-hybrid screen. These include the Drosophila FGF-receptor Heartless and Dof itself. We show that the integrity of the DBB motif is required both for Dof and for BCAP to form dimers. Analysis of the interactions between a set of deletion constructs of Dof and the panel of interactors suggests that Dof may adopt different conformations, with a folded conformation stabilized by interactions between the DBB motif and the C-terminal part of the protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Binding Sites
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Carrier Proteins / chemistry*
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Carrier Proteins / metabolism
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Cells, Cultured
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Conserved Sequence*
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Dimerization
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Drosophila / chemistry
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Drosophila / genetics
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Drosophila / metabolism
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Drosophila Proteins*
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Gene Expression Regulation, Developmental
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In Situ Hybridization
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Insect Proteins / chemistry*
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Insect Proteins / genetics
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Insect Proteins / metabolism*
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Membrane Proteins / chemistry
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Protein Binding
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Protein Structure, Tertiary
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Fibroblast Growth Factor / metabolism
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Signal Transduction*
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Two-Hybrid System Techniques
Substances
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Adaptor Proteins, Signal Transducing
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BANK1 protein, human
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Carrier Proteins
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Drosophila Proteins
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Insect Proteins
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Membrane Proteins
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PIK3AP1 protein, human
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RNA, Messenger
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Receptors, Fibroblast Growth Factor
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stumps protein, Drosophila