Independent recruitment in vivo by Gal4 of two complexes required for transcription

Mol Cell. 2003 May;11(5):1301-9. doi: 10.1016/s1097-2765(03)00144-8.


We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.

MeSH terms

  • Binding Sites / genetics
  • Cells, Cultured
  • DNA-Binding Proteins
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation, Fungal / genetics*
  • Genes, Regulator / genetics*
  • Macromolecular Substances
  • Mediator Complex
  • Promoter Regions, Genetic / genetics*
  • RNA Polymerase II / genetics
  • Reaction Time / genetics
  • Saccharomyces cerevisiae Proteins / genetics*
  • Trans-Activators / genetics
  • Transcription Factors / genetics*
  • Transcriptional Activation / genetics*
  • Yeasts / genetics*


  • DNA-Binding Proteins
  • GAL11 protein, S cerevisiae
  • GAL4 protein, S cerevisiae
  • Macromolecular Substances
  • Mediator Complex
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • RNA Polymerase II