The Saccharomyces cerevisiae bud-neck proteins Kcc4 and Gin4 have distinct but partially-overlapping cellular functions

Genes Genet Syst. 2003 Apr;78(2):113-26. doi: 10.1266/ggs.78.113.

Abstract

In the budding yeast S. cerevisiae, Swe1 delays the onset of mitosis by phosphorylation and inactivation of the cyclin-dependent kinase Cdc28, thereby relaying the morphogenetic signal to the cell cycle. Hsl1/Nik1, Kcc4 and Gin4 are structurally homologous protein kinases that localize to the bud neck and negatively regulate Swe1 by phosphorylation. We report here that Kcc4 and Gin4 have partially overlapping but essentially distinct cellular functions. Deletion of KCC4 had a similar effect to GIN4 deletion, causing moderate defects in bud formation at stationary phase; overexpression of Kcc4 inhibited cell growth. KCC4 showed functional interaction with GIN4 in cdc28 mutants, and both Kcc4 and Gin4 proteins physically interacted with Swe1 in vitro. However, unlike gin4delta cells, kcc4Delta cells were not elongated but multi-budded at stationary phase, and showed resistance to 0.04% SDS and 0.003% calcofluor white. In light of the observation that Kcc4 and Gin4 specifically associate with distinct septin proteins, we propose that the observed functional distinction between Kcc4 and Gin4 is due to differences in septin association partners.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzenesulfonates / metabolism
  • CDC28 Protein Kinase, S cerevisiae / genetics
  • CDC28 Protein Kinase, S cerevisiae / metabolism
  • Cell Cycle / physiology
  • Cell Cycle Proteins
  • Cell Division / physiology*
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / physiology*
  • Mutation
  • Protein Kinases / genetics
  • Protein Kinases / physiology*
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Sodium Dodecyl Sulfate / metabolism
  • Structural Homology, Protein
  • Transcription, Genetic

Substances

  • Benzenesulfonates
  • Cell Cycle Proteins
  • Saccharomyces cerevisiae Proteins
  • Sodium Dodecyl Sulfate
  • C.I. Fluorescent Brightening Agent 28
  • Protein Kinases
  • SWE1 protein, S cerevisiae
  • Protein-Tyrosine Kinases
  • GIN4 protein, S cerevisiae
  • KCC4 protein, S cerevisiae
  • CDC28 Protein Kinase, S cerevisiae
  • Cyclin-Dependent Kinases