Stimulation of c-MYC transcriptional activity and acetylation by recruitment of the cofactor CBP

EMBO Rep. 2003 May;4(5):484-90. doi: 10.1038/sj.embor.embor821.


The c-MYC oncoprotein regulates various aspects of cell behaviour by modulating gene expression. Here, we report the identification of the cAMP-response-element-binding protein (CBP) as a novel c-MYC binding partner. The two proteins interact both in vitro and in cells, and CBP binds to the carboxy-terminal region of c-MYC. Importantly, CBP, as well as p300, is associated with E-box-containing promoter regions of genes that are regulated by c-MYC. Furthermore, c-MYC and CBP/p300 function synergistically in the activation of reporter-gene constructs. Thus, CBP and p300 function as positive cofactors for c-MYC. In addition, c-MYC is acetylated in cells. This modification does not require MYC box II, suggesting that it is independent of TRRAP complexes. Instead, CBP acetylates c-MYC in vitro, and co-expression of CBP with c-MYC stimulates in vivo acetylation. Functionally, this results in a decrease in ubiquitination and stabilization of c-MYC proteins. Thus, CBP and p300 are novel functional binding partners of c-MYC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl Coenzyme A / metabolism
  • Acetylation
  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism*
  • Adaptor Proteins, Signal Transducing
  • Binding Sites
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cells, Cultured
  • Cyclic AMP Response Element-Binding Protein / chemistry
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • Genes, Reporter
  • Histone Acetyltransferases
  • Humans
  • Nuclear Proteins / metabolism
  • Proto-Oncogene Proteins c-myc / chemistry
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Transcription Factors
  • Transcription, Genetic
  • Transcriptional Activation
  • p300-CBP Transcription Factors


  • Adaptor Proteins, Signal Transducing
  • Cell Cycle Proteins
  • Cyclic AMP Response Element-Binding Protein
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-myc
  • Recombinant Fusion Proteins
  • Transcription Factors
  • transformation-transcription domain-associated protein
  • Acetyl Coenzyme A
  • Acetyltransferases
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor