Deficiency of CD26 results in a change of cytokine and immunoglobulin secretion after stimulation by pokeweed mitogen

Eur J Immunol. 2003 Jun;33(6):1519-27. doi: 10.1002/eji.200323469.


To investigate the role of CD26 in the immune system, CD26 gene knockout mice with C57BL/6 background were used to study the immune response after stimulation with PWM. CD26(-/-) mice display an apparently normal phenotype. However, in their spleen lymphocyte population the percentage of CD4(+) T cells is lower, and that of NK cells is higher, than that in CD26(+/+) mice. In their peripheral blood, CD26(-/-) mice present a conspicuously decreased proportion of CD4(+) NKT lymphocytes. In vitro, the PWM-stimulated IL-4 production was decreased by 60-80% in the supernatants of spleen lymphocytes of CD26(-/-) mice compared to that of CD26(+/+) mice, whereas levels of IL-10 and IFN-gamma were increased. No significant differences were found in the production of IL-2, IL-5, IL-6 and IL-13 between knockout and wild-type mice. After immunization of mice with PWM in vivo, serum levels of total IgG, IgG1, IgG2a and IgE were markedly lower in CD26(-/-) mice than those in CD26(+/+) mice, while no difference was found in IgM production. Further analysis of cytokine levels in vivo revealed a reduced IL-4, IL-2 and delayed IFN-gamma production in sera of CD26(-/-) mice upon immunization with PWM. These results indicate that CD26 contributes to the regulation of development, maturation and migration of CD4(+) T, NK and NKT cells, cytokine secretion, T cell-dependent antibody production and immunoglobulin isotype switching of B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • Concanavalin A / pharmacology
  • Dipeptidyl Peptidase 4 / genetics
  • Dipeptidyl Peptidase 4 / physiology*
  • Female
  • Immunization
  • Immunoglobulin Class Switching / physiology*
  • Immunoglobulin E / blood
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Immunoglobulins / blood*
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / blood
  • Interferon-gamma / metabolism
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Interleukins / biosynthesis
  • Interleukins / blood
  • Interleukins / metabolism
  • Killer Cells, Natural / classification
  • Killer Cells, Natural / immunology
  • Lymphocyte Activation / drug effects*
  • Lymphokines / biosynthesis
  • Lymphokines / blood
  • Lymphokines / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Pokeweed Mitogens / pharmacology*
  • Specific Pathogen-Free Organisms
  • Spleen / cytology
  • Spleen / immunology


  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulins
  • Interleukin-2
  • Interleukins
  • Lymphokines
  • Pokeweed Mitogens
  • Concanavalin A
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma
  • Dipeptidyl Peptidase 4